#The importance of serial dilution serial number
#The importance of serial dilution series

If the dilution factor of the first tube, r = 10-1 (1 ml added to 9 ml) and the dilution factor of the second tube= 10-1 (1ml added to 9 ml), then, Total dilution factor for the second tube = dilution of first tube × dilution of the second tube Note: After the first tube, each tube is the dilution of the previous dilution tube.

#The importance of serial dilution series

The dilution factor of single tubes within a series In serial dilution technique, the dilution factor can be calculated either for a single test tube or for the entire series (total dilution factor). In laboratories two-fold and ten-fold dilution is used to titer antibodies or prepare diluted analytes. In contrast, if the source of the sample is less polluted then a low dilution factor should be sufficient. For example, if a sample is collected from a highly polluted source, then the dilution factor will be increased. The extension of dilution factor or the number of serial dilution is increased based on the concentration cells present within the unknown sample. After that, a small amount of sample from each dilution is used to prepare a series of pour or spread plates. In serial dilution, the selected sample is diluted through a set of standard volumes of sterile diluents, such as be distilled water or 0.9 % saline. Serial dilution formula – serial dilution calculation The incubated plates from the serial dilution generate an easily countable number of colonies, hence we can easily enumerate the number of cells present within the sample. It also used to avoid having to pipette very small volumes (1-10 µl) to make a dilution of a solution. The main purpose of serial dilution technique is to find out the concentration or the cell counts of an anonymous sample by counting the number of colonies that are cultured from the serial dilutions of the sample. This makes it easier to calculate the cell numbers in the primary solution by calculating the total dilution over the whole series.

#The importance of serial dilution serial number

In serial dilution, the cell count or density gradually decreases as the serial number increases in each step. In the laboratory, this method is used to decrease the counts of cells within a culture to simplify the operation. In a single and very simple word, Serial dilution is a laboratory technique, in which a stepwise dilution process is performed on a solution with an associated dilution factor. Hey there, you might be thinking, what is serial dilution?Īs the term indicates, it is a series of succeeding dilutions that performed to create a less dense or less concentrated solution from a high dense or concentrated solution. What is a serial dilution? – serial dilution definition